Some Biochemical Changes in Carrot Roots Induced by Erwinia carotovora var. carotovora
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Biological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species
Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovo...
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The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
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The purified obelin was electrophoresed on 7 % polyacrylamide gels in Tris-glycine buffer, pH 8.3, containing 0.1 mM-EDTA. Gels stained with Coomassie Brilliant Blue showed a single band at the buffer front, which correlated in position with luminescent activity. This activity could be eluted from unstained gel slices by soaking them overnight in 0.1 mM-EDTA-lOmM-Hepes, pH 7.0, at 4°C. Obelin i...
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The physicochemical properties of the antileukemic Lasparaginase from Erwinia carotovora have been studied and compared with those of the corresponding clinically effective enzyme from Escherichia coli B. Although both enzymes are tetramers with similar molecular weight, the amino acid compositions are distinctly different and the Erwinia enzyme is more basic as judged by polyacrylamide electro...
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We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid ana...
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ژورنال
عنوان ژورنال: HortScience
سال: 2006
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.41.4.989d